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Journal: bioRxiv

Article Title: Genetic Ablation and Multi-Omics Profiling Reveal CEP55 as a Key Driver of Tumorigenesis in Diverse Cancer Models

doi: 10.1101/2025.10.28.685219

Figure Lengend Snippet:

Article Snippet: Tissues and cells were lysed in RIPA or urea buffers, protein quantified via BCA assay, electrophoresed via SDS-PAGE, transferred to nitrocellulose membranes, and probed with antibodies listed: CEP55 CST (D1L4H) Rabbit mAb #81693 1:1000; Cep55 Santa Cruz biotechnology, sc-374051, 1:500; Vinculin Cell Signaling Technology #13901, 1:2000; β-Actin BD Pharmingen#612656, 1:2000; β-Catenin Cell Signaling Technology 9582, 1:1000; pERK1/2 (T202/Y204) Cell Signaling Technology #4370, 1:1000; ERK1/2Cell Signaling Technology #4695, 1:1000; pAKTS473 Cell Signaling Technology #4060, 1:1000; AKT Cell Signaling Technology #9271, 1:1000; MYC (Y69) Abcam #ab32072, 1:1000; Rabbit Secondary (peroxidase) Sigma Aldrich #A0545, 1:5000; Mouse Secondary (peroxidase) Sigma Aldrich #A9044, 1:5000.

Techniques: Expressing

Journal: bioRxiv

Article Title: Genetic Ablation and Multi-Omics Profiling Reveal CEP55 as a Key Driver of Tumorigenesis in Diverse Cancer Models

doi: 10.1101/2025.10.28.685219

Figure Lengend Snippet:

Article Snippet: Tissues and cells were lysed in RIPA or urea buffers, protein quantified via BCA assay, electrophoresed via SDS-PAGE, transferred to nitrocellulose membranes, and probed with antibodies listed: CEP55 CST (D1L4H) Rabbit mAb #81693 1:1000; Cep55 Santa Cruz biotechnology, sc-374051, 1:500; Vinculin Cell Signaling Technology #13901, 1:2000; β-Actin BD Pharmingen#612656, 1:2000; β-Catenin Cell Signaling Technology 9582, 1:1000; pERK1/2 (T202/Y204) Cell Signaling Technology #4370, 1:1000; ERK1/2Cell Signaling Technology #4695, 1:1000; pAKTS473 Cell Signaling Technology #4060, 1:1000; AKT Cell Signaling Technology #9271, 1:1000; MYC (Y69) Abcam #ab32072, 1:1000; Rabbit Secondary (peroxidase) Sigma Aldrich #A0545, 1:5000; Mouse Secondary (peroxidase) Sigma Aldrich #A9044, 1:5000.

Techniques: Expressing